p re ss hepg2 cells Search Results


p berghei liver stage phenotyping hepg2 human hepatoma cells  (ATCC)
99
ATCC p berghei liver stage phenotyping hepg2 human hepatoma cells
P Berghei Liver Stage Phenotyping Hepg2 Human Hepatoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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compound 2 for  (ATCC)
99
ATCC compound 2 for
Compound 2 For, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hepg2-cyp3a4 transfected cells  (Yoshitomi Pharmaceutical Industries)
90
Yoshitomi Pharmaceutical Industries hepg2-cyp3a4 transfected cells
Hepg2 Cyp3a4 Transfected Cells, supplied by Yoshitomi Pharmaceutical Industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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incubation with g418  (Thermo Fisher)
99
Thermo Fisher incubation with g418
Incubation With G418, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dpx2 cells  (Puracyp Inc)
90
Puracyp Inc dpx2 cells
(A) Quantification of BDP metabolites produced by A549 cells treated with BDP or BDP with esterase inhibitors (+EI). (B) Relative quantification of BDP metabolites produced by <t>DPX2</t> cells treated with BDP or BDP with esterase inhibitors (+EI). Data are the mean and standard deviation from six replicates. n.d. Signifies that the metabolite was not detected. (C and D) <t>CYP3A</t> enzyme mRNA abundance, measured by qPCR in A549 (C) and DPX2 (D) cells. Data are represented as the number of mRNA copies per 10,000 copies of β2-macroglobulin (a “housekeeping” gene). Statistics used for A549 cell data analysis were one-way analysis of variance with Dunnett’s post-hoc test. For DPX2 cell data analysis two-way ANOVA with Bonferronni post-hoc testing was used. Data are the mean and standard deviation from 6 replicates. n.d. Signifies that mRNA was not detected. *P < 0.05; ***P < 0.001; ****P < 0.0001.
Dpx2 Cells, supplied by Puracyp Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleospin rna ii kit  (MACHEREY NAGEL)
96
MACHEREY NAGEL nucleospin rna ii kit
(A) Quantification of BDP metabolites produced by A549 cells treated with BDP or BDP with esterase inhibitors (+EI). (B) Relative quantification of BDP metabolites produced by <t>DPX2</t> cells treated with BDP or BDP with esterase inhibitors (+EI). Data are the mean and standard deviation from six replicates. n.d. Signifies that the metabolite was not detected. (C and D) <t>CYP3A</t> enzyme mRNA abundance, measured by qPCR in A549 (C) and DPX2 (D) cells. Data are represented as the number of mRNA copies per 10,000 copies of β2-macroglobulin (a “housekeeping” gene). Statistics used for A549 cell data analysis were one-way analysis of variance with Dunnett’s post-hoc test. For DPX2 cell data analysis two-way ANOVA with Bonferronni post-hoc testing was used. Data are the mean and standard deviation from 6 replicates. n.d. Signifies that mRNA was not detected. *P < 0.05; ***P < 0.001; ****P < 0.0001.
Nucleospin Rna Ii Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aryl hydrocarbon receptor ahr activation  (InvivoGen)
95
InvivoGen aryl hydrocarbon receptor ahr activation
(A) Quantification of BDP metabolites produced by A549 cells treated with BDP or BDP with esterase inhibitors (+EI). (B) Relative quantification of BDP metabolites produced by <t>DPX2</t> cells treated with BDP or BDP with esterase inhibitors (+EI). Data are the mean and standard deviation from six replicates. n.d. Signifies that the metabolite was not detected. (C and D) <t>CYP3A</t> enzyme mRNA abundance, measured by qPCR in A549 (C) and DPX2 (D) cells. Data are represented as the number of mRNA copies per 10,000 copies of β2-macroglobulin (a “housekeeping” gene). Statistics used for A549 cell data analysis were one-way analysis of variance with Dunnett’s post-hoc test. For DPX2 cell data analysis two-way ANOVA with Bonferronni post-hoc testing was used. Data are the mean and standard deviation from 6 replicates. n.d. Signifies that mRNA was not detected. *P < 0.05; ***P < 0.001; ****P < 0.0001.
Aryl Hydrocarbon Receptor Ahr Activation, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hepg2 cell line  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection hepg2 cell line
Glucose‐regulated protein 78 (GRP78) engineering enhanced yields of TfR‐Ab and improved cell viability. Chinese hamster ovary (CHO) and CHO modified by GRP78 (CHO‐GRP78) cells were seeded in 12‐well plates at a density of 2 × 105 cells per well and transiently transfected with pOptiVEC™‐TOPO®/TfR‐Ab, followed by culturing with SFM4CHO™ medium. (A) The concentration of TfR‐Ab in the supernatant was detected by ELISA assay. (B) The quality of TfR‐Ab in the supernatant was evaluated by its binding ability with TfR+ <t>HepG2</t> cells using flow cytometry (FCM). The bar graphs represented FCM analysis of mean fluorescence index (MFI). (C) Bar graph represented FCM analysis of the percentage viability of CHO and CHO‐GRP78 cells. (D) The viable cell numbers of CHO and CHO‐GRP78 cells were counted after Trypan blue staining. Data were mean values ± SD, n > 3.*p < 0.05,**p < 0.01,***p < 0.001 versus negative control.
Hepg2 Cell Line, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cancer cell lines  (ATCC)
99
ATCC human cancer cell lines
Glucose‐regulated protein 78 (GRP78) engineering enhanced yields of TfR‐Ab and improved cell viability. Chinese hamster ovary (CHO) and CHO modified by GRP78 (CHO‐GRP78) cells were seeded in 12‐well plates at a density of 2 × 105 cells per well and transiently transfected with pOptiVEC™‐TOPO®/TfR‐Ab, followed by culturing with SFM4CHO™ medium. (A) The concentration of TfR‐Ab in the supernatant was detected by ELISA assay. (B) The quality of TfR‐Ab in the supernatant was evaluated by its binding ability with TfR+ <t>HepG2</t> cells using flow cytometry (FCM). The bar graphs represented FCM analysis of mean fluorescence index (MFI). (C) Bar graph represented FCM analysis of the percentage viability of CHO and CHO‐GRP78 cells. (D) The viable cell numbers of CHO and CHO‐GRP78 cells were counted after Trypan blue staining. Data were mean values ± SD, n > 3.*p < 0.05,**p < 0.01,***p < 0.001 versus negative control.
Human Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cancer cell lines - by Bioz Stars, 2026-05
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doxorubicin  (MedChemExpress)
96
MedChemExpress doxorubicin
Glucose‐regulated protein 78 (GRP78) engineering enhanced yields of TfR‐Ab and improved cell viability. Chinese hamster ovary (CHO) and CHO modified by GRP78 (CHO‐GRP78) cells were seeded in 12‐well plates at a density of 2 × 105 cells per well and transiently transfected with pOptiVEC™‐TOPO®/TfR‐Ab, followed by culturing with SFM4CHO™ medium. (A) The concentration of TfR‐Ab in the supernatant was detected by ELISA assay. (B) The quality of TfR‐Ab in the supernatant was evaluated by its binding ability with TfR+ <t>HepG2</t> cells using flow cytometry (FCM). The bar graphs represented FCM analysis of mean fluorescence index (MFI). (C) Bar graph represented FCM analysis of the percentage viability of CHO and CHO‐GRP78 cells. (D) The viable cell numbers of CHO and CHO‐GRP78 cells were counted after Trypan blue staining. Data were mean values ± SD, n > 3.*p < 0.05,**p < 0.01,***p < 0.001 versus negative control.
Doxorubicin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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doxorubicin - by Bioz Stars, 2026-05
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hepg 2  (DSMZ)
96
DSMZ hepg 2
Glucose‐regulated protein 78 (GRP78) engineering enhanced yields of TfR‐Ab and improved cell viability. Chinese hamster ovary (CHO) and CHO modified by GRP78 (CHO‐GRP78) cells were seeded in 12‐well plates at a density of 2 × 105 cells per well and transiently transfected with pOptiVEC™‐TOPO®/TfR‐Ab, followed by culturing with SFM4CHO™ medium. (A) The concentration of TfR‐Ab in the supernatant was detected by ELISA assay. (B) The quality of TfR‐Ab in the supernatant was evaluated by its binding ability with TfR+ <t>HepG2</t> cells using flow cytometry (FCM). The bar graphs represented FCM analysis of mean fluorescence index (MFI). (C) Bar graph represented FCM analysis of the percentage viability of CHO and CHO‐GRP78 cells. (D) The viable cell numbers of CHO and CHO‐GRP78 cells were counted after Trypan blue staining. Data were mean values ± SD, n > 3.*p < 0.05,**p < 0.01,***p < 0.001 versus negative control.
Hepg 2, supplied by DSMZ, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hep g2 cells  (JCRB Cell Bank)
90
JCRB Cell Bank hep g2 cells
Glucose‐regulated protein 78 (GRP78) engineering enhanced yields of TfR‐Ab and improved cell viability. Chinese hamster ovary (CHO) and CHO modified by GRP78 (CHO‐GRP78) cells were seeded in 12‐well plates at a density of 2 × 105 cells per well and transiently transfected with pOptiVEC™‐TOPO®/TfR‐Ab, followed by culturing with SFM4CHO™ medium. (A) The concentration of TfR‐Ab in the supernatant was detected by ELISA assay. (B) The quality of TfR‐Ab in the supernatant was evaluated by its binding ability with TfR+ <t>HepG2</t> cells using flow cytometry (FCM). The bar graphs represented FCM analysis of mean fluorescence index (MFI). (C) Bar graph represented FCM analysis of the percentage viability of CHO and CHO‐GRP78 cells. (D) The viable cell numbers of CHO and CHO‐GRP78 cells were counted after Trypan blue staining. Data were mean values ± SD, n > 3.*p < 0.05,**p < 0.01,***p < 0.001 versus negative control.
Hep G2 Cells, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Quantification of BDP metabolites produced by A549 cells treated with BDP or BDP with esterase inhibitors (+EI). (B) Relative quantification of BDP metabolites produced by DPX2 cells treated with BDP or BDP with esterase inhibitors (+EI). Data are the mean and standard deviation from six replicates. n.d. Signifies that the metabolite was not detected. (C and D) CYP3A enzyme mRNA abundance, measured by qPCR in A549 (C) and DPX2 (D) cells. Data are represented as the number of mRNA copies per 10,000 copies of β2-macroglobulin (a “housekeeping” gene). Statistics used for A549 cell data analysis were one-way analysis of variance with Dunnett’s post-hoc test. For DPX2 cell data analysis two-way ANOVA with Bonferronni post-hoc testing was used. Data are the mean and standard deviation from 6 replicates. n.d. Signifies that mRNA was not detected. *P < 0.05; ***P < 0.001; ****P < 0.0001.

Journal: The Journal of Pharmacology and Experimental Therapeutics

Article Title: Metabolism of Beclomethasone Dipropionate by Cytochrome P450 3A Enzymes

doi: 10.1124/jpet.112.202556

Figure Lengend Snippet: (A) Quantification of BDP metabolites produced by A549 cells treated with BDP or BDP with esterase inhibitors (+EI). (B) Relative quantification of BDP metabolites produced by DPX2 cells treated with BDP or BDP with esterase inhibitors (+EI). Data are the mean and standard deviation from six replicates. n.d. Signifies that the metabolite was not detected. (C and D) CYP3A enzyme mRNA abundance, measured by qPCR in A549 (C) and DPX2 (D) cells. Data are represented as the number of mRNA copies per 10,000 copies of β2-macroglobulin (a “housekeeping” gene). Statistics used for A549 cell data analysis were one-way analysis of variance with Dunnett’s post-hoc test. For DPX2 cell data analysis two-way ANOVA with Bonferronni post-hoc testing was used. Data are the mean and standard deviation from 6 replicates. n.d. Signifies that mRNA was not detected. *P < 0.05; ***P < 0.001; ****P < 0.0001.

Article Snippet: DPX2 cells (HepG2 background with human PXR stably overexpressed to drive the expression of a CYP3A4 reporter gene construct by PXR agonists) were provided by Dr. Judy Raucy (Puracyp Inc., Carlsbad, CA).

Techniques: Produced, Quantitative Proteomics, Standard Deviation

Glucose‐regulated protein 78 (GRP78) engineering enhanced yields of TfR‐Ab and improved cell viability. Chinese hamster ovary (CHO) and CHO modified by GRP78 (CHO‐GRP78) cells were seeded in 12‐well plates at a density of 2 × 105 cells per well and transiently transfected with pOptiVEC™‐TOPO®/TfR‐Ab, followed by culturing with SFM4CHO™ medium. (A) The concentration of TfR‐Ab in the supernatant was detected by ELISA assay. (B) The quality of TfR‐Ab in the supernatant was evaluated by its binding ability with TfR+ HepG2 cells using flow cytometry (FCM). The bar graphs represented FCM analysis of mean fluorescence index (MFI). (C) Bar graph represented FCM analysis of the percentage viability of CHO and CHO‐GRP78 cells. (D) The viable cell numbers of CHO and CHO‐GRP78 cells were counted after Trypan blue staining. Data were mean values ± SD, n > 3.*p < 0.05,**p < 0.01,***p < 0.001 versus negative control.

Journal: Engineering in Life Sciences

Article Title: Overexpression of GRP78 enhances survival of CHO cells in response to serum deprivation and oxidative stress

doi: 10.1002/elsc.201500152

Figure Lengend Snippet: Glucose‐regulated protein 78 (GRP78) engineering enhanced yields of TfR‐Ab and improved cell viability. Chinese hamster ovary (CHO) and CHO modified by GRP78 (CHO‐GRP78) cells were seeded in 12‐well plates at a density of 2 × 105 cells per well and transiently transfected with pOptiVEC™‐TOPO®/TfR‐Ab, followed by culturing with SFM4CHO™ medium. (A) The concentration of TfR‐Ab in the supernatant was detected by ELISA assay. (B) The quality of TfR‐Ab in the supernatant was evaluated by its binding ability with TfR+ HepG2 cells using flow cytometry (FCM). The bar graphs represented FCM analysis of mean fluorescence index (MFI). (C) Bar graph represented FCM analysis of the percentage viability of CHO and CHO‐GRP78 cells. (D) The viable cell numbers of CHO and CHO‐GRP78 cells were counted after Trypan blue staining. Data were mean values ± SD, n > 3.*p < 0.05,**p < 0.01,***p < 0.001 versus negative control.

Article Snippet: Cell culture The Chinese hamster ovary (CHO) cells, human stomach adenocarcinoma (AGS) cell line, and human hepatoma cell line HepG2 (China Center for Type Culture Collection, Wuhan, P. R. China) were cultured in Roswell Park Memorial Institute (RPMI) 1640 and DMEM medium (Gbico BRL, NY, USA) supplemented with 10% FBS (Sijiqing, Hangzhou, China) and 100 U/mL ampicillin, 100 mg/mL streptomycin in an incubator containing 5% CO 2 at 37°C (standard conditions).

Techniques: Modification, Transfection, Concentration Assay, Enzyme-linked Immunosorbent Assay, Binding Assay, Flow Cytometry, Fluorescence, Staining, Negative Control